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Nanorulers from the GATTA-PAINT HiRes series serve all advantages of the classical GATTA-PAINT nanorulers. Additionally， they include special DNA-PAINT based fiducial markers for a precise and reliable drift correction. These fiducial markers outclass common fiducial markers based on fluorescent beads by having a stable connection to the surface and emitting a signal which does neither bleach nor exceed the detector’s dynamic range.
For the GATTA-PAINT HiRes nanorulers we offer the mark-to-mark distances in the sizes 20 nm,， 40 nm and 80 nm. Each size is available in the colors red (ATTO 655) or green (ATTO 542).
On request we can also design special solutions for your specific requirements. All samples will be delivered on a sealed glass slide which you can comfortably put directly on your microscope.
GATTA-PAINT HiRes 80R | GATTA-PAINT HiRes 80G | |
顏色 | 紅 | 綠 |
熒光染料 | ATTO 655 | ATTO 542 |
激光源 | 625 - 665 nm | 530 - 560 nm |
表面染料密度 Fiducial Marker | ≈ 0,，1/μm2 | ≈ 0,，1/μm2 |
表面染料密度 PAINT 納米標尺 | ≈ 1/μm2 | ≈ 1/μm2 |
PAINT 納米標尺 間 距 | 2x 80 nm | 2x 80 nm |
水平 | Beginner | Beginner |
GATTA-PAINT HiRes 40R | GATTA-PAINT HiRes 40G | |
顏色 | 紅 | 綠 |
熒光染料 | ATTO 655 | ATTO 542 |
激光源 | 625 - 665 nm | 530 - 560 nm |
表面染料密度 Fiducial Marker | ≈ 0,，1/μm2 | ≈ 0，1/μm2 |
表面染料密度 PAINT 納米標尺 | ≈ 1/μm2 | ≈ 1/μm2 |
PAINT 納米標尺 間 距 | 2x 40 nm | 2x 40 nm |
水平 | Advanced | Advanced |
GATTA-PAINT HiRes 20R | GATTA-PAINT HiRes 20G | |
顏色 | 紅 | 綠 |
熒光染料 | ATTO 655 | ATTO 542 |
激光源 | 625 - 665 nm | 530 - 560 nm |
表面染料密度 Fiducial Marker | ≈ 0,，1/μm2 | ≈ 0，1/μm2 |
表面染料密度 PAINT 納米標尺 | ≈ 1/μm2 | ≈ 1/μm2 |
PAINT 納米標尺 間 距 | 2x 20 nm | 2x 20 nm |
水平 | Expert | Expert |
Beginner:Samples to check the basic functions of your microscope. Easy to resolve,， also for beginners in the field of super-resolution microscopy.
Advanced: Samples to optimize the settings of your microscope. Advanced experience is necessary in order to resolve the nanorulers.
Expert: Samples which demand perfectly aligned microscopes and very experienced users which are able to optimize acquisition and data evaluation.
熒光團 | 顏色定義 | 激發(fā)波長/nm | 發(fā)射波長/nm |
ATTO 655 | R | 655 | 680 |
ATTO 542 | G | 542 | 562 |
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