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LaVision BioTec UltraMicroscope II 光片掃描顯微鏡
快速,、大樣本、3D全方位成像
從組織器官整體水平探究生物系統(tǒng)的機(jī)理,,是當(dāng)前生命科學(xué)研究的另一前沿領(lǐng)域,。 LaVision BioTec UltraMicroscope II光片顯微鏡采用對(duì)偶正交光通路,,能夠?qū)⒐庹障拗圃谝粋€(gè)平面上,為研究者從整體上研究生物系統(tǒng)和生理過程提供了全新的工具,。LaVision BioTec現(xiàn)已加入德國(guó)美天旎Miltenyi Biotec家族,。
優(yōu)化的光照支持大樣本成像
?雙向各三束光片令光照均一,**程度避免照射大樣本時(shí)常見的暗帶和投影帶問題(見圖1A,、B),;
?大樣本腔、大視場(chǎng)和長(zhǎng)達(dá)5200 mm的工作距離和可完成對(duì)如成體斑馬魚,、嚙齒動(dòng)物胚胎的3D掃描成像;
**靈活的掃描技術(shù)
?可通過軟件界面靈活調(diào)節(jié)光片參數(shù)及光照方式,,不論整體概覽還是高分辨率特寫,同樣達(dá)到**成像效果(見圖2A),;
?水平光路動(dòng)態(tài)聚焦技術(shù),,確保了對(duì)大體積樣本或高倍放大中**的Z軸分辨率(見圖2B);
?不同波長(zhǎng)發(fā)射光經(jīng)修正聚焦于同一平面,,支持多通道圖像清晰疊加,;
智能強(qiáng)大的分析軟件
?支持?jǐn)?shù)據(jù)疊加和多種智能算法,以實(shí)現(xiàn)**呈現(xiàn)效果的3D圖像,;
?支持對(duì)不同透明化試劑和成像浸入液的折射率補(bǔ)償和修正,;
操作便捷、用戶友好
?**一款支持所有組織透明化方案的鏡頭,,無論水溶性緩沖液和還是基于有機(jī)溶劑,,實(shí)驗(yàn)設(shè)計(jì)不受局限;
?從儀器校準(zhǔn),、日常維護(hù)到實(shí)際操作,,僅需簡(jiǎn)單培訓(xùn)即可輕松上手;
LaVision UltraMicroscope II已被眾多學(xué)者引用于學(xué)術(shù)期刊:
Panicle-Shaped Sympathetic Architecture in the
Spleen Parenchyma Modulates Antibacterial Innate Immunity
Xiaofan Ding et al. Cell Reports (2019):27,, 3799–3807
Contemporaneous 3D characterization of acute
and chronic myocardial I/R injury and response
Simon F. Merz et al. Nat. Commun. (2019) 2312
A network of trans-cortical capillaries as mainstay for blood circulation in long bones.
Grüneboom,, A. et al.
Nature Metabolism (2019) 1: 236–250.
Immunolabeling of cleared human pancreata
provides insights into three-dimensional
pancreatic anatomy and pathology.
No?, M. et al.
Am. J. Path. (2018) 188:1530–1535.
Matrix stiffness controls lymphatic vessel
formation through regulation of a GATA2-
dependent transcriptional program.
Frye,, M. et al.
Nat. Commun. (2018) 9: 1511.
GABAergic inhibition in dual-transmission
cholinergic and GABAergic striatal interneurons is abolished in Parkinson disease.
Lozovaya,, N. et al.
Nat. Commun. (2018) 9: 1422.
VIPAR, a quantitative approach to 3D
histopathology applied to lymphatic
malformations.
H?gerling,, R. et al.
JCI Insight. (2017) 2: e93424.
Mapping of brain activity by automated volume
analysis of immediate early genes.
Renier,, N. et al. Cell (2016) 165: 1789–1802.
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