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The VenaT4™ biochip is the only biochip available for studying chemotaxis， transmigration and invasion assays and is Cellix's new microflow transwell chamber.

VenaT4™趨化性研究芯片是Cellix推出的一種新型Transwell室,，可以用來進(jìn)行趨化（chemotaxis）,、遷移(transmigration)、侵襲（invasion）研究,。

The VenaT4TM biochip model combines Cellix's proprietary microfluidic flow technology for cell recruitment under shear stress with traditional transwell chamber models. Used to investigate and quantify cell recruitment to various ligands in the presence of chemoattractants contained within the microwells mimicking tissue and / or injury sites.

趨化性研究芯片結(jié)合Cellix專有的微流體技術(shù),，用于研究在剪切力下細(xì)胞的聚集。利用微孔模擬組織和/或傷口,，微孔包覆化學(xué)引誘物,，用于研究和量化細(xì)胞對(duì)各種配體的聚集。

The biochips contain 4 microcapillaries in parallel,， each with an embedded microporous membrane with pores of 2-10um diameter (depending on application). Underneath each membrane is a microwell. See image above. The microcapillaries of the VenaT4TMbiochips are easily coated with ECM proteins using a standard yellow-tip pipette,， giving the researcher greater flexibility. The VenaT4TM biochip is suitable for the following assays:

Chemotaxis Assays: Cells flowing through the microcapillary of the biochip may attach to the protein coated membrane and migrate towards the chemokine in the underlying microwell.

趨化性實(shí)驗(yàn)：細(xì)胞流經(jīng)微流道，可能與膜上的蛋白涂布粘附,，然后向底層的趨化因子遷移,。

Transmigration Assays: Cells flowing through the microcapillary of the biochip may attach to the cell monolayer on the membrane and transmigrate through the cell monolayer and membrane towards the chemokine in the underlying microwell.

遷移實(shí)驗(yàn)：細(xì)胞流經(jīng)微流道，可能與膜上的單個(gè)細(xì)胞粘附,，然后穿過細(xì)胞核膜,，向底層的趨化因子遷移。

Invasion Assays: Cells flowing through the microcapillary of the biochip may attach to the cell monolayer on the membrane and transmigrate through the cell monolayer and membrane into a 3D ECM in the underlying microwell.

侵襲實(shí)驗(yàn)：細(xì)胞流經(jīng)微流道,，可能與膜上的單個(gè)細(xì)胞粘附,，然后穿過細(xì)胞核膜，向底層的3D ECM遷移,。

The VenaT4TM biochip is suitable for use with the following:

Cell types: Leukocytes,， neutrophils， lymphocytes,， macrophages,， monocytes， endothelial cells,， epithelial cells,， fibroblasts and tumour cells.

細(xì)胞種類：白細(xì)胞、中性粒細(xì)胞,、淋巴細(xì)胞,、巨噬細(xì)胞、單核細(xì)胞,、內(nèi)皮細(xì)胞,、上皮細(xì)胞、成纖維細(xì)胞和腫瘤細(xì)胞,。

Coatings: collagen,， fibronectin,， laminin etc.

涂布：膠原、纖連蛋白,、層粘連蛋白等

產(chǎn)品特點(diǎn)：

* 適合白細(xì)胞和癌癥細(xì)胞的遷移,、侵襲和趨化性實(shí)驗(yàn)（migration， transmigration,， invasion and chemotax）

* 可以進(jìn)行全血和血細(xì)胞分析（如白細(xì)胞）

* 聚碳酸酯膜,，空隙大小為2-10μm。

* 每個(gè)芯片有4個(gè)微流道,，每個(gè)容量?jī)H14μL,。可以將化學(xué)引誘物固定在基質(zhì)膠（ECM gel）里面

* 配合Kima泵使用,，可進(jìn)行長(zhǎng)期的遷移研究

* 利用MirusTM Nanopump可以提供并控制0.05–200 dyne/cm2的流體剪切力,，剪切流可為脈沖流或平穩(wěn)流。

* 在流體剪切力環(huán)境下,，實(shí)時(shí)成像,、實(shí)時(shí)觀察

* 可使用明場(chǎng)/相稱/熒光顯微鏡，20x,， 40x長(zhǎng)工作距離顯微鏡下觀察,，芯片光學(xué)性能良好

請(qǐng)關(guān)注Cellix公司獨(dú)特的微流體技術(shù)：

* 全面的微流體方案，提供*接近生理環(huán)境的流體剪切力

* Lab on a Chip,，節(jié)省樣本,，可實(shí)時(shí)觀察

* 細(xì)胞粘附,、遷移,、趨化性研究的新手段，新思路

剪切力細(xì)胞分析系統(tǒng)

剪切力細(xì)胞分析系統(tǒng)能提供接近生理血流狀態(tài)下的剪切流,，逼真模擬人體生理環(huán)境,，適用于細(xì)胞滾動(dòng)、粘附和遷移的研究,，并且能夠?qū)崿F(xiàn)高通量細(xì)胞分析,。目前已廣泛應(yīng)用于心血管疾病、腫瘤,、傳染性疾病,、呼吸道、免疫學(xué)及細(xì)菌學(xué)等領(lǐng)域,。