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細(xì)胞微流體工作站VenaFlux (VenaFlux Platforms)是Cellix公司目前*受客戶歡迎的一套系統(tǒng),,提供在活細(xì)胞水平上微流體活性篩選的完整解決方案。操作簡(jiǎn)單,,分析效率高,,可大大提高藥品的開發(fā)和篩選效率。
VenaFlux 系統(tǒng)是**個(gè)能進(jìn)行連續(xù)流體細(xì)胞分析的半自動(dòng)化微流體分析系統(tǒng),。配合使用Cellix生物芯片和細(xì)胞分析軟件,,VenaFlux 系統(tǒng)可檢測(cè)粘附到微毛細(xì)管上的細(xì)胞,微毛細(xì)管中覆蓋抗體或者培養(yǎng)有內(nèi)皮細(xì)胞,,模擬生理流動(dòng)產(chǎn)生一定的剪切力,。
VenaFlux 系統(tǒng)操作簡(jiǎn)單,可減少耗時(shí)耗錢的動(dòng)物模型實(shí)驗(yàn),,獲得特異性強(qiáng),,準(zhǔn)確性高,重復(fù)性好的結(jié)果,。
VenaFlux 流體細(xì)胞分析儀產(chǎn)品特點(diǎn):
? 廣泛使用于各種細(xì)胞懸浮液,;
? 生物芯片可選擇用光學(xué)顯微鏡做細(xì)節(jié)研究;
? 微流體泵可方便的控制流體剪切力在0.05到20dyne/cm2范圍內(nèi),;
? 在分析中可調(diào)整剪切力,;
? 流體速度可控制在pL/min到mL/min;
? 可做nL到mL 的樣品分析,;
? 使用DucoCell 分析軟件(DucoCell Analysis Software),,可簡(jiǎn)單快速的調(diào)節(jié)好顯微鏡的各項(xiàng)參數(shù);
? 統(tǒng)計(jì)分析生成的圖表(如:細(xì)胞粘附性VS.剪切力)可方便的導(dǎo)入Excel文檔,;
? 電子機(jī)械臂移動(dòng)生物芯片,,清洗細(xì)微組件和稀釋細(xì)胞樣品;
? VenaFluxTM 系統(tǒng)的軟件同步調(diào)節(jié)泵,,翻譯階段篩選,,圖像采集,樣品移動(dòng)和流體控制,,各項(xiàng)操作界面均是用戶友好型,,系統(tǒng)先進(jìn)又易于操作;
細(xì)胞微流體工作站 (VenaFlux Platforms)的主要技術(shù)參數(shù):
Microscope Axiovert 40:
Contrast option: Brightfield,, Phase,, Varel.
Objectives: 10x LD A-Plan, 20x LD A-Plan, 32x CP-Achromat,, 40x LD A-Plan
Eyepieces: E-PL 10x/20 Br.,, 10x/20 Br. foc.
Stage: Vena8 biochip compatible stage holder, Eppendorf tubes holder.
Mirus 1.0 Nanopump
Specifications: Shear Stress Range of 0.05–20 dyne/cm2,,
Volumetric Flow Rates 100nL–20μL/minute (100μL syringe),,
Sample Volume Increments Freely adjustable Linear Velocity Range of 10 μm/s to 10 cm/s;
Flow Direction Reversible;
Sample Volume Aspiration Accuracy ±1% ;
Shear Stress Accuracy ±0.5%;
Sample Volume Aspiration Precision < 1%.
Multichannel: 16-Way manifold allowing individual addressing microchannels of Vena8 biochip.
Microscope Cage incubator:
Capabilities: Temperature control ± 0.10C, CO2 and humidity module.
Options: Temperature monitoring software module,, black panels for use in fluorescent microscopy.
Deltapix DP 200 USB 2.0 camera
Specifications: 1.3 Mega Pixels (1,,280 x 1,024),, Color CDD,, 1/2" format, Pixel 5.2 μm x 5.2 μm,,
Resolution: 15 FPS @ 1,,280 x 1,024 pixels; 24 FPS @ 1,,204 x 768 pixels; 37 FPS @ 800 x 600 pixels; 60 FPS @ 640 x 480 pixels. Dynamic Range > 60 dB.
Dell Optiplex 745 PC computer
Configuration: Intel Core2 processor; 1.86 GHz; 2GB RAM; ATI Radeon X1300PRO;
Objective Imaging stage control hardware; 19” LCD monitor.
Windows XP Professional SP2;
DeltaPix Viewer Software;
FlowAssay and DucoCell software preinstalled.
FlowAssay Software.
Capabilities: Execution of preset protocols.
Intuitive stepwise protocol interface with steps hierarchy and status control.
DucoCell software
Specifications: Automatic Counting and Detection;
Morphological parameters analyzed automatically(Area,, diameter, form-factor,, ellipticity,, eccentricity, orientation,, perimeter,, asymmetry,, cell centroid coordinates,, elliptical axis);
Cell sorting and filtering;
Ability to analyze sub-populations based on inclusion/exclusion of morphological parameters.
Data may be exported to Excel spreadsheet incorporating automatic graph generation (e.g. % cell adhesion vs. shear stress).
File Formats analyzed*.jpg, *.jpeg,, *.tif,, *.tiff, *.bmp,, *.gif,, *.png.
Vena8 biochips pack of 10
Specifications: Minimum Sample Volume 3 μL;
Maximum Sample Volume 100 μL; Shear Stress Range of 0.05– 20 dyne/cm2, steps of 0.05dyne/cm2.
Number of channels per biochip is 8;
Volume of each channel is 0.8μL.
Dead volume at input port is 0.1 μL. Vena8 biochip dimensions are 400 μm (W) x 100 μm (D) x 20 mm(L).
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