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人體血液循環(huán)模擬系統(tǒng)，VenaFlux 微流體細(xì)胞研究系統(tǒng) Automated microfluidic platform是提供在活細(xì)胞水平上微流體活性篩選的完整解決方案,。操作簡單,，分析效率高，可大大提高藥品的開發(fā)和篩選效率,。

VenaFlux 系統(tǒng)是**個(gè)能進(jìn)行連續(xù)流體細(xì)胞分析的半自動(dòng)化微流體分析系統(tǒng),。配合使用Cellix生物芯片和細(xì)胞分析軟件，VenaFlux 系統(tǒng)可檢測(cè)粘附到微毛細(xì)管上的細(xì)胞,，微毛細(xì)管中覆蓋抗體或者培養(yǎng)有內(nèi)皮細(xì)胞,，模擬生理流動(dòng)產(chǎn)生一定的剪切力。

VenaFlux 系統(tǒng)操作簡單,，可減少耗時(shí)耗錢的動(dòng)物模型實(shí)驗(yàn),，獲得特異性強(qiáng)，準(zhǔn)確性高,，重復(fù)性好的結(jié)果,。

VenaFlux流體細(xì)胞分析儀產(chǎn)品特點(diǎn)：

廣泛使用于各種細(xì)胞懸浮液；

生物芯片可選擇用光學(xué)顯微鏡做細(xì)節(jié)研究,；

微流體泵可方便的控制流體剪切力在0.05-200 Dyne/cm2范圍內(nèi),；

在分析中可調(diào)整剪切力；

流體速度可控制在pL/min到mL/min,；

可做nL到mL 的樣品分析,；

使用DucoCell 分析軟件（DucoCell Analysis Software），可簡單快速的調(diào)節(jié)好顯微鏡的各項(xiàng)參數(shù),；

統(tǒng)計(jì)分析生成的圖表（如：細(xì)胞粘附性VS.剪切力）可方便的導(dǎo)入Excel文檔,；

電子機(jī)械臂移動(dòng)生物芯片，清洗細(xì)微組件和稀釋細(xì)胞樣品,；

VenaFlux系統(tǒng)的軟件同步調(diào)節(jié)泵,，翻譯階段篩選，圖像采集,，樣品移動(dòng)和流體控制,，各項(xiàng)操作界面均是用戶友好型，系統(tǒng)先進(jìn)又易于操作,；

主要配置方案：

一,、Partial Platform -STARTER-1.0：

1、Mirus Nanopump 1.0

2,、1 pack VenaEC biochips

3,、1 pack Vena8 biochips

4、Frame for VenaEC biochips

5,、DucoCell Software ver 1.1

二,、Microfluidic SP1.0 (Basic full Package)-MICROSP-1.0：

1,、arl Zeiss Axiovert 40 microscope， CCD Deltapix DP200 camera,， Dell PC,， Microscope cage incubator

2、Mirus Nanopump 1.0 inc. VenaFluxAssay software

3,、1 pack Vena8 biochips

4,、 1 pack VenaEC biochips

5、DucoCell Software ver 1.1

三,、CMicrofluidic SP1.1-MICROSP-1.1：

1,、Carl Zeiss Axiovert 40 CFL microscope， CCD Deltapix DP450 camera,， Dell PC,， Microscope cage incubator， Fluorescent and brightfield shutters ,，

2,、Mirus Nanopump 1.0 inc. VenaFluxAssay software

3、1 pack Vena8 biochips

4,、1 pack VenaEC biochips

5,、DucoCell Software ver 1.1

四、Microfluidic SP1.2-MICROSP-1.2:

1,、Carl Zeiss Axiovert 40 CFL microscope,， CCD Deltapix DP450 camera， Dell PC,， Microscope cage incubator,， Fluorescent and brightfield shutters,，motorised

stage

2,、Mirus Nanopump 1.0 inc. VenaFluxAssay software

3、1 pack VenaEC biochips

4,、1 pack Vena8 biochips

5,、DucoCell Software ver 1.1

五、VenaFlux-VENAFLUX-1.0：

1,、Carl Zeiss Axiovert 40 CFL microscope,， CCD Deltapix DP450 camera， Dell PC,， Microscope cage incubator,， Fluorescent and brightfield shutters

2、VenaFluxAssay software plus automatic image capture

3,、Robotic sample handler

4,、Mirus Nanopump 1.1 inc. 8-way Manifold

5,、1 pack VenaEC biochips

6、1 pack Vena8 biochips

7,、Frame for VenaEC biochips

8,、DucoCell Software ver 1.1

主要技術(shù)參數(shù)：

Microscope Axiovert 40:

Contrast option: Brightfield， Phase,， Varel.

Objectives: 10x LD A-Plan,， 20x LD A-Plan， 32x CP-Achromat,， 40x LD A-Plan

Eyepieces: E-PL 10x/20 Br.,， 10x/20 Br. foc.

Stage: Vena8 biochip compatible stage holder， Eppendorf tubes holder.

Mirus 1.0 Nanopump

Specifications: Shear Stress Range of 0.05–20 dyne/cm2,，

Volumetric Flow Rates 100nL–20μL/minute (100μL syringe),，

Sample Volume Increments Freely adjustable Linear Velocity Range of 10 μm/s to 10 cm/s;

Flow Direction Reversible;

Sample Volume Aspiration Accuracy ±1% ;

Shear Stress Accuracy ±0.5%;

Sample Volume Aspiration Precision < 1%.

Multichannel: 16-Way manifold allowing individual addressing microchannels of Vena8 biochip.

Microscope Cage incubator:

Capabilities: Temperature control ± 0.10C， CO2 and humidity module.

Options: Temperature monitoring software module,， black panels for use in fluorescent microscopy.

Deltapix DP 200 USB 2.0 camera

Specifications: 1.3 Mega Pixels (1,，280 x 1，024),， Color CDD,， 1/2" format， Pixel 5.2 μm x 5.2 μm,，

Resolution: 15 FPS @ 1,，280 x 1，024 pixels; 24 FPS @ 1,，204 x 768 pixels; 37 FPS @ 800 x 600 pixels; 60 FPS @ 640 x 480 pixels. Dynamic Range > 60 dB.

Dell Optiplex 745 PC computer

Configuration: Intel Core2 processor; 1.86 GHz; 2GB RAM; ATI Radeon X1300PRO;

Objective Imaging stage control hardware; 19” LCD monitor.

Windows XP Professional SP2;

DeltaPix Viewer Software;

FlowAssay and DucoCell software preinstalled.

FlowAssay Software.

Capabilities: Execution of preset protocols.

Intuitive stepwise protocol interface with steps hierarchy and status control.

DucoCell software

Specifications: Automatic Counting and Detection;

Morphological parameters analyzed automatically(Area,， diameter， form-factor,， ellipticity,， eccentricity， orientation,， perimeter,， asymmetry， cell centroid coordinates,， elliptical axis);

Cell sorting and filtering;

Ability to analyze sub-populations based on inclusion/exclusion of morphological parameters.

Data may be exported to Excel spreadsheet incorporating automatic graph generation (e.g. % cell adhesion vs. shear stress).

File Formats analyzed*.jpg,， *.jpeg， *.tif,， *.tiff,， *.bmp， *.gif,， *.png.

Vena8 biochips pack of 10

Specifications: Minimum Sample Volume 3 μL;

Maximum Sample Volume 100 μL; Shear Stress Range of 0.05– 20 dyne/cm2,， steps of 0.05dyne/cm2.

Number of channels per biochip is 8;

Volume of each channel is 0.8μL.

Dead volume at input port is 0.1 μL. Vena8 biochip dimensions are 400 μm (W) x 100 μm (D) x 20 mm(L).

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